WebOct 26, 2024 · The localization pattern of Unc5 protein was quite similar to that of Fra, with localization in the lamina glial cells visualized using repo-Gal4 UAS-CD8GFP (Figure 1F2; white arrows). Note that repo-Gal4 UAS-CD8GFP visualizes the glial cell membrane, whereas Repo antibody visualizes the glial cell nuclei, because Repo is a nuclear protein. WebPlasmid ZP30 Fgf8 from Dr. Randall Moon's lab contains the insert fgf8. This plasmid is available through Addgene.
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WebTo generate pG80,y+, the white gene from pG80,w+ was. excised by EcoRV/SacII digestion, the vector was blunted with Klenow, and replaced with the. yellow gene as a blunted SalI fragment from the y S/G plasmid … WebNov 15, 2003 · The subpopulation of these embryos homozygous for the RN2-GAL4,UAS-CD8GFP third chromosome (which gave a stronger GFP signal than RN2-GAL4,UAS-CD8GFP/TM3) was used for the analysis. For all experiments using a CyO balancer chromosome (below), the progeny not carrying this chromosome (negative for wg - lacZ … buy diamond dotz
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WebAug 25, 2006 · Summary The Hippo signaling pathway acts upon the Yorkie transcriptional activator to control tissue growth in Drosophila. Activated Yorkie drives growth by stimulating cell proliferation and inhibiting apoptosis, but how it achieves this is not understood. Yorkie is known to activate Cyclin E (CycE) and the apoptosis inhibitor DIAP1. WebCD8GFP, can mark both ISC and enteroblasts in adult Drosoph-ila midgut (Figure 1C). We used these flies for feeding experi-ments with 3% of the various polysaccharides in the … WebCD8GFP and Su(H)Gbe-LacZ had increased by approximately 4-fold, while Delta- and Prospero-positive cell number had changed by less than 1.5-fold (Figure 1P). These results support our idea that the most prominent phenotype induced by DSS is an accumulation of enteroblasts. Intestinal Stem Cell Division Is Increased after DSS Feeding cell phone repair feather banners