Csfv shimen strain

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August undefined, 2024

WebApr 2, 2024 · A multiplex reverse transcription polymerase chain rection (mRT-PCR) was developed for simultaneous detection of four RNA viruses in swine. The conserved target sequences directed to classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV) and … WebCSFV infection induced mitogen-activated protein kinase activation, but did not elicit NFκB activation, thereby affecting the production of pro-inflammatory cytokines. The Shimen …

A multiplex RT-PCR assay for rapid and simultaneous detection of four ...

WebMar 9, 2010 · For the specificity assay, the RNA from the cells infected with CSFV strains (C-strain, Shimen, QZ-07, and XS-08), PCV2, PRRSV, PRV, or mock-infected cells was extracted for the established RT-nPCR. Three independent experiments were performed to test the repeatability. Sensitivity was determined through serial dilutions of in vitro … WebJul 19, 2024 · Classical swine fever virus (CSFV) is a major animal pathogen threatening the global pork industry. To date, numerous anti-CSFV monoclonal antibodies (mAbs) and their recognizing epitopes have been reported. However, few mAbs were systematically characterized for the capacity to differentiate field CSFV isolates from CSF vaccine … how to remove ear block

Efficacy evaluation of the C-strain-based vaccines against the ...

WebDocument: In a combined vaccine approach, SFV DNA vectors and recombinant adenovirus expressing the classical swine fever virus (CSFV) E2 glycoprotein elicited higher titers of neutralizing antibodies in pigs [26] . After challenges with the virulent CSFV Shimen strain, no symptoms of viremia were observed, for the combined vaccine, whereas vaccination … WebDuplex PCR for distinguishing shimen and C-strain of CSFV A duplex PCR for distinguishing the Shimen strain from the C-strain was performed with a total volume of … WebJun 25, 2024 · The CSFV Shimen strain, a highly virulent strain isolated in China in 1945, were also used as a reference challenge virus in the present study. Primer design and RT-PCR. Six pairs of primers based on the published known sequence of CSFV Zj0801 (GenBank accession no. FJ529205) were designed for PCR analysis (Table ... how to remove ear fluid

Self-Assembling Nanovaccine Enhances Protective Efficacy Against CSFV …

WebSep 13, 2013 · CSFV Shimen strain was used as challenge virus in this study and PAMs were cultured in vitro. Interferon regulatory factor (IRF)-3 and nuclear factor-kappa B (NF-κB) translocation was detected using immunofluorescent staining; RIG-I, MDA5, interferon promoter-stimulating factor 1 (IPS-1), IRF-3 and NF-κB expression was measured by … WebMay 31, 2024 · A full-length cDNA clone of attenuated strain HuN4-F112, obtained by serial cell passage from HP-PRRSV HuN4-F5 (GenBank accession no. EF635006), was used as the backbone for insertion of the E2 gene from the CSFV Shimen strain (GenBank accession no. AF092448) [24]. The resultant recombinant virus was compared to the … how to remove ear piercing hoop with ballWebJul 21, 2024 · All the vaccinated piglets were challenged intramuscularly with 10 5 TCID 50 CSFV Shimen strain at 35 dpi. For single dose immunization schedule, twelve 4-week-old pigs were randomly divided into three groups (A, B and C, n = 4), and subject to an immunization and challenge trial. In brief, Groups A and B were respectively immunized … how to remove ear pain

"WebDec 1, 2013 · The strains of CSFV (Shimen and C) used were obtained from the Control Institute of Veterinary Bioproducts and Pharmaceuticals (China). Virus strains were … " - Csfv shimen strain

Csfv shimen strain

Differentiation of virulent Shimen and vaccine C …

WebApr 23, 2014 · The CSFV Shimen and C strains were obtained from the Control Institute of Veterinary Bioproducts and Pharmaceuticals (Beijing, China). SUVECs were cultured in 25-cm 2 tissue culture flasks at a density of 2 × 10 7 cells per flask. The Shimen and C strains of CSFV were added to cultures at an MOI of 10 TCID50 per cell when SUVECs were … WebNov 29, 2012 · In the present study, CSFV Shimen strain full-length genomic cDNA carrying a genetic tag (AvaI site) was cloned downstream of the cytomegalovirus (CMV) …

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WebSep 8, 2014 · The virulent Shimen strain of CSFV, which is the standard virulent strain that has been used for vaccine potency tests in China since the 1950s, was used in this … WebJul 22, 2024 · The CSFV Shimen strain (GenBank accession number AF092248.2) was generated from a cDNA clone (pSPT I /SM) and propagated in PK15 cells. The BVDV I Hubei strain (GenBank accession number MZ484396) is a passaged variant of BVDV I NDAL strain (GenBank accession number AJ133738.1) [ 19 ] and propagated in MDBK …

WebMar 8, 2024 · The Shimen strain of classical swine fever (CSF) virus (CSFV) causes CSF, which is mainly characterised by disseminated intravascular haemorrhage. Macrophages … WebNov 19, 2024 · Then, the cells were stimulated with ultraviolet-light-inactivated CSFV (Shimen strain; MOI = 1) l. After 24 h of incubation at 37 °C, the cell-free supernatant was removed and detected using a commercially available porcine IL-4 and IFN-γ ELISA kit according to the manufacturer’s protocol. OD values were measured at 450 nm using a ...

WebSep 13, 2013 · In present study, we investigated the roles of RIG-I and MDA-5 in eliciting antiviral and inflammatory responses to CSFV shimen strain in Porcine alveolar … Web猪瘟病毒野毒株E2蛋白特异性单抗免疫抑制制备法的建立.pdf

WebJul 1, 2015 · The Shimen strain infection resulted in a significant activation of IFN regulatory factor IRF7 and suppression of IRF3. ... two classical swine fever virus (CSFV) strains, namely the highly virulent CSFV Shimen strain and the avirulent C strain (a vaccine strain), were employed, and the expression of 19 immune effector genes was …

WebClassical swine fever (CSF) caused by the classical swine fever virus (CSFV) has resulted in severe losses to the pig industry worldwide. It has been proposed that lipid synthesis is essential for viral replication, and lipids are involved in viral protein maturation and envelope production. However, the specific crosstalk between CSFV and host cell lipid metabolism … how to remove ear plugs from earWebMar 14, 2024 · The Shimen strain is the standard virulent strain of CSFV in China , and induces severe immunopathological symptoms associated with hemorrhagic fever, thrombocytopenia, and lymphoid organ atrophy and … how to remove earring backWebAug 17, 2024 · In this study, we demonstrated that P108 and T109 on the E2 glycoprotein together with the E rns glycoprotein of the rabbit-adaptive C-strain confer adaptation to rabbits on the highly virulent CSFV Shimen strain by affecting viral entry during infection but do not attenuate the Shimen strain in pigs. Our results provide vital information on ... how to remove earrings with ball backsWebThe classical swine fever virus (CSFV) C-strain has been used as a vaccine strain for over 60 years in China. A recent study has demonstrated that the E2 protein of C-strain plays a major role in... how to remove ear piercing with flat backWebA Biblioteca Virtual em Saúde é uma colecao de fontes de informacao científica e técnica em saúde organizada e armazenada em formato eletrônico nos países da Região Latino-Americana e do Caribe, acessíveis de forma universal na Internet de modo compatível com as bases internacionais. how to remove earringsWebMar 8, 2024 · The Shimen strain of classical swine fever virus (CSFV) causes an especially infectious disease in domestic pigs known as classical swine fever (CSF), which has been listed as a highly contagious disease by the World Organisation for Animal Health [].Acute CSF is typically accompanied by haemorrhagic lymphadenitis and diffuse haemorrhage … how to remove ear tags from sheep how to remove earrings that are stuck