WebDAPI Fluoromount-G ® is a water-soluble, 4',6-diamidino-2-phenylindole (DAPI) containing compound for slides mounted after a staining procedure having an aqueous final step. It … WebFluoromount ™ Aqueous Mounting Medium has been used for mounting: coverslips on slides for immunofluorescence microscopic examination of PC12 and Hela cells; …

Upregulation of astroglial connexin 30 impairs hippocampal …

WebCells were fixed in 4% w/v PFA, washed in 50 mM PIPES buffer, pH 7.4 before mounting with Fluoromount ™ (Sigma Aldrich) on poly-lysine coated slides. ThT fluorescence (green) is depicted ... WebFor Sigma-Aldrich products where long-term storage is advised as refrigerator/cooler, our labels indicate 2 to 8 oC. This matches the recommended range indicated in the USP as refrigerator (36 deg F to 46 deg F). Controlled Freezer For Sigma-Aldrich products where long-term storage is advised as freezer, our labels indicate -20 oC. smart board 7000 series

Fluoromount ™ Aqueous Mounting Medium - Sigma-Aldrich

WebDec 23, 2015 · Finally, cells were stained with DAPI nucleic acid stain (4′,6-diamidino-2-phenylindole, Invitrogen, Carlsbad, CA, USA) and mounted using Fluoromount mounting medium (Sigma). Samples were analyzed using the Leica TCS SP5 confocal laser-scanning microscope (Leica, Wetzlar, Germany), and the resulting images were processed using … WebFluoroshield with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This mounting medium is fortified with DAPI which is a counterstain for DNA. For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. WebApr 11, 2024 · Upregulation of connexin 30 (Cx30) in astrocytes increases the connectivity of astroglial networks, but decreases spontaneous and evoked synaptic transmission, altering the induction of synaptic plasticity and in vivo learning processes, and suggesting that astroglial networks may have a physiologically optimized size to appropriately … hill of beans quote casablanca