Hoechst 33342 toxicity
Nettet4. sep. 2024 · However, it quickly became evident that exposure to membrane-permeant DNA fluorophores, such as Hoechst 33342 and DRAQ5, induce distinct DNA damage responses in cells, including activation of ATM, Chk2 and p53 [ 6 ]. Furthermore, repeated exposure to UV light is particularly damaging to cells as it causes photolysis of DNA [ 7 ]. NettetToxicity Toxic, harmful Health Hazards Wear PPE at all times, handle with glove. Potential Hazards CNS and Liver; harmful by skin absorption, irritant mutagen. Carcinogenicity: No significant acute toxicological data identified AnaSpec Inc. 34801 Campus Drive, Fremont, CA 94555 Toll-Free: 800-452-5530 • Tel: 510-791-9560 • Fax: …
Hoechst 33342 toxicity
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Nettet6. mai 2024 · Answer Hoechst is minimally toxic to cells especially when used at very low concentrations. It is the preferred dye for staining live cells because of its lower … Nettet10. aug. 2016 · Combining Lipinski’s rule with the docking and steered molecular dynamics simulations and using the PubChem data base of about 1.4 million compounds, we have obtained DNA dyes Hoechst 34580 and Hoechst 33342 as top-leads for the Alzheimer’s disease. The binding properties of these ligands to amyloid beta (Aβ) fibril were …
Nettet28. jan. 2014 · The kinetics of cell loss and the rather large dose modification factor suggests that Hoechst 33342 may be suppressing an apoptotic response in this subpopulation. Whatever the mechanism involved, Hoechst 33342 clearly provides substantial protection against early radiation-induced endothelial cell loss. Nettet28. jul. 2024 · Red Vybrant Tongue (V22885; Thermo Fisher Scientific Orange Red) was used to stain entire cells and Hoechst 33342 (H3570; Thermo Fisher Scientific) (3:1000) was used to stain nuclei. Following 10 minutes of staining, CurcuEmulsomes were added to the culture and images were taken in series for 15 minutes from different Z-stacks …
NettetHoechst 33342染色常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。 Hoechst 33342也常用于普通的细胞核染色，或常规的DNA染色。 Hoechst 33342的最大激发波长为346nm，最大发射波长为460nm；Hoechst 33342和双链DNA结合后，最大激发波长为350nm，最大发射波长为461nm。 Nettet30. jun. 2016 · In the current study, we discovered that Hoechst 33342 (H342) could produce significant toxicity to the human neuroblastoma SK-N-SH cells at …
Nettet1. aug. 1986 · The inherent toxicity of Hoechst 33342 and in particular its preferential toxicity to treated cells poses a significant limita tion on the use of this dye in …
Nettet1. jul. 1986 · Hoechst 33342 stain has been used previously in conjunction with fluorescence activated cell sorting to separate cell populations into the various phases … merlin age seven deadly sinsNettetDisplaying low-cytotoxic effects and increased cell permeability, Hoechst stains are ideal for live- and fixed-cell fluorescent DNA staining and nuclei imaging techniques, such as cell counting, automated DNA determination, cell cycle analysis, chromosome sorting, and nuclear counterstaining. merlina gacha clubNettetIt is quite toxic to some cell types and potentiates may toxins bacuse it shares xenobiotic drug pumps. The BEST way to use Hoechst33342 dye is to make up a stock in DMSO … merlina informacionNettet1. okt. 2010 · In this study we demonstrate that the common live-cell DNA stain Hoechst 33342 can cause apoptosis under CTLM conditions. Although toxicity is evident at … merlin aeronauticsNettetI'm planning to stain fixed Vero nuclei with Hoechst 33342. The protocol manual provides quite a range for both concentration (0.2 - 2 µg/ml) and incubation time (1 - 15 min) when staining... how post-it notes were inventedNettet12. apr. 2024 · Nuclei were stained with Hoechst 33342 (1/1000). Anti-endomucin antibody (sc-65495, Santa Cruz, diluted 1/200) was used to stain vessels in bone marrow and spleen. how postman tool worksNettetCell cycle specific toxicity of the Hoechst 33342 stain in untreated or irradiated murine tumor cells. Hoechst 33342 stain has been used previously in conjunction with … merlin aggregates doncaster