Witryna(HCT-0100, Immunovision, 1:500) and an Alexa Flour 555 goat anti-human secondary antibody (A21433, 1:500, Invitrogen, Life Technologies). Vectashield with DAPI (H-1200, Vector Laboratories) was used as a mounting medium. The cells were scanned using a Leica AF 6000 inverted fluorescence microscope, equipped with a HCX PL APO … WitrynaFigure Legend Snippet: A − C Asynchronous cells were stained with Hoechst, CREST, and anti-Aurora B-pT232 or anti-Aurora B antibodies. In total, 90 kinetochores from 30 …
小儿体外循环肺损伤的研究 - 豆丁网
Witryna14 wrz 2024 · Cells were placed on the slides treated with the indicated concentrations for 72 h and followed fixation with 4% paraformaldehyde in PBS for 20 min at room temperature, permeabilized with 5% Triton X-100 in PBS for 30 min. Blocked cells with 3% bovine serum albumin (BSA) were incubated with primary antibodies γ-H2AX (Cell … WitrynaACA Immunovision HCT-0100 1:5000 Pericentrin Abcam Ab4448 1:750 Plk1 Santa Cruz SC-17783 1:1000 1:1000 Flag Sigma F1804 1:500 dsRed Clontech 632496 1:1000 Cyclin B1 BD Biosciences 554177 1:2000 pS170-Cyk4 Home brewed (Burkard et al., 2009) 1:500 Mklp1 Santa Cruz SC-867 1:350 ... solar web portal
Human Autoimmune Serum Erba Diagnostics Bioz
Witryna16 gru 2012 · Timely dissolution of sister-chromatid cohesion in mitosis ensures accurate chromosome segregation to guard against aneuploidy and tumorigenesis. The … WitrynaHematokryt (HCT) to badanie, które pozwala określić, jaki procent całej objętości krwi stanowią krwinki czerwone. Zarówno obniżony, jak i podwyższony poziom … WitrynaThe number and quality of oocytes, as well as the decline in both of these parameters with age, determines reproductive potential in women. However, the underlying mechanisms of this diminution are incompletely understood. Previously, we identified novel roles for CHTF18 (Chromosome Transmission Fidelity Factor 18), a component … solar websites