Web20 Mar 2012 · First, Parkin translocation onto mitochondria is slower than in nonneuronal cells. Second, Parkin is selectively recruited to depolarized mitochondria to form ring-like … WebTranslocation of pre-protein domains across the IM requires ATP-driven pre-sequence translocase-associated motor (PAM). A small number of IM proteins are encoded by …

Parkin mitochondrial translocation is achieved through a …

Web12 Sep 2014 · In fact, in the PARKIN-induced mitophagy pathway, PARKIN, an E3-ubiquitin ligase, translocates from the cytosol to mitochondria in cells treated with CCCP. 5, 7 After translocation to the ... WebWhen probing the mechanism, increased Parkin translocation to the mitochondria may be responsible for the up-regulated mitophagy exerted by melatonin. Parkin knockout … pinkpop holland

Parkin regulates microglial NLRP3 and represses …

In a recent study, a Parkin peptide array was used to map the binding region for its own UBL domain32. Interestingly, two motifs, one in RING0 and the other in the RBR region (the exact positions are not provided in the report) were found to have ubiquitin-binding capacity. This observation prompted us to test … See more Interestingly, when Pink1ΔMTS was co-expressed with Parkin, an extra higher molecular weight species, ∼8 kDa above the main band, was detected for Parkin C431S but not Parkin WT (Figure 1B). Parkin C431S also … See more As Parkin C431S can trap ubiquitin and still bind to Pink1ΔMTS, we inferred that the overall structure of Parkin is intact in this mutant. One intriguing interpretation of the loss of … See more WebTaken together with the translocation kinetics of PARKIN mutants, these data provide direct quantitative evidence of a multistep feedforward mechanism involving (1) … Web15 Jan 2024 · The Parkinson's disease-associated protein kinase PINK1 and ubiquitin ligase Parkin coordinate the ubiquitination of mitochondrial proteins, which marks mitochondria … pinkpop crash